Exploring the Influence of Mucin on Lipid Bilayers: Insights into Phase-Dependent Interactions

Changes in the behavior of the prepared lipid bilayer according to each phase of the layer, induced by mucin incorporation, were investigated. 8-Aminonaphtalene-1,3,6-trisulfonic acid disodium salt (ANTS) was encapsulated only in the water-soluble interior of the vesicle. p-xylene-bis(N-pyridinium bromide) (DPX) settled only outside of the vesicles to quench the ANTS. The calibration was established for the fluorescence with the encapsulated ANTS separated from the DPX (100%) and the ANTS mixed with DPX (0%). The changes were found due to the layer phase over the ratio of mucin to lipid. The changes are determined by the stability of the vesicles.