Isolation of Escherichia Coli from Clinical Mastitis Cases of Cows in Nangarhar Province

Mastitis is a common inflammation of the mammary glands caused by a variety of microbial and non-microbial factors and one of the major agent is Escherichia coli (E. coli) bacteria. The disease affects lactating cow’s production and bear economic consequences. The purpose of this study is to isolate E. coli bacteria from infected mammary glands and the prevalence of E. coli mastitis in lactating cows in Nangarhar province. In total 50 cow milk samples were collected from different districts of veterinary clinics, villages and dairy farms of Nangarhar province. The samples were examined with gram staining and catalase tests for diagnosis. The results revealed that 21 (42%) cows were infected with E. coli and 29 (58%) were infected by various susceptible microorganisms. In summary, considering the massive number of cultured E. coli bacteria, the research suggested that E. coli infection is highly ranked in Nangarhar different areas and could cause economic losses in cow bio productions.